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vectorshield antifade mounting medium with dapi  (Vector Laboratories)


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    Vector Laboratories vectorshield antifade mounting medium with dapi
    Vectorshield Antifade Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 22036 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vectorshield+with+dapi/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/10__1016_slash_j__nbd__2025__107156-81-20-26
    Average 98 stars, based on 22036 article reviews
    vectorshield antifade mounting medium with dapi - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Staining:

    Article Title: Complement activation on neutrophils initiates endothelial adhesion and extravasation
    Article Snippet: Cytospin samples were blocked with the anti-FcR mAb (1:100 dilution, cat#101302, BioLegend) in 5% BSA in PBS buffer for 1 hour at RTand probed with anti-mouse IgG (1:100 dilution, cat#115-295-164, Jackson ImmunoReseach) and biotinylated anti-mouse myeloperoxidase (MPO, 1:100 dilution, cat# {"type":"entrez-protein","attrs":{"text":"S22223","term_id":"95048","term_text":"pir||S22223"}} S22223 , Hycult Biotech) and for 2 hours at RT followed by streptavidin Alexa Fluor 488. .. Vectorshield with DAPI (cat#H-1200, Vector Laboratories) was used to stain nuclei. ..

    Negative Control:

    Article Title: The Landscape of Telomere Length and Telomerase in Human Embryos at Blastocyst Stage
    Article Snippet: Then, blastocysts were incubated in an anti-hTERT antibody (ThermoFisher Scientific, Waltham, MA, USA, Cat# PA5116024) solution diluted with blocking buffer in a 1 to 200 ratio at 37 °C for 1 h. Two aneuploid blastocysts were incubated with blocking buffer without the anti-hTERT antibody to serve as a negative control. .. All blastocysts, including the negative control blastocysts, were washed 3 times in blocking buffer for a total of 30 min, and then incubated with Goat anti-Rabbit IgG 2 nd antibody (ThermoFisher Scientific, Rockford, IL, USA, Cat#35552) diluted 1:400 in blocking buffer at 37 °C for 30 min. After the second antibody incubation, blastocysts were washed three times in blocking buffer for 15 min. Immunostained blastocysts were loaded on a pre-cleaned glass slide with 15 μL of VectorShield with DAPI (Vector Laboratories, Newark, CA, USA, Cat# H-1200-10) and sealed with a coverslip and clear nail polish. .. Images were taken with a Zeiss microscope equipped with epifluorescence optics.

    Article Title: The Landscape of Telomere Length and Telomerase in Human Embryos at Blastocyst Stage.
    Article Snippet: Then, blastocysts were incubated in an anti-hTERT antibody (ThermoFisher Scientific, Waltham, MA, USA, Cat# PA5116024) solution diluted with blocking buffer in a 1 to 200 ratio at Genes 2023, 14, 1200 4 of 12 37 ◦C for 1 h. Two aneuploid blastocysts were incubated with blocking buffer without the anti-hTERT antibody to serve as a negative control. .. All blastocysts, including the negative control blastocysts, were washed 3 times in blocking buffer for a total of 30 min, and then incubated with Goat anti-Rabbit IgG 2nd antibody (ThermoFisher Scientific, Rockford, IL, USA, Cat#35552) diluted 1:400 in blocking buffer at 37 ◦C for 30 min. After the second antibody incubation, blastocysts were washed three times in blocking buffer for 15 min. Immunostained blastocysts were loaded on a pre-cleaned glass slide with 15 μL of VectorShield with DAPI (Vector Laboratories, Newark, CA, USA, Cat# H-1200-10) and sealed with a coverslip and clear nail polish. .. Images were taken with a Zeiss microscope equipped with epifluorescence optics.

    Blocking Assay:

    Article Title: The Landscape of Telomere Length and Telomerase in Human Embryos at Blastocyst Stage
    Article Snippet: Then, blastocysts were incubated in an anti-hTERT antibody (ThermoFisher Scientific, Waltham, MA, USA, Cat# PA5116024) solution diluted with blocking buffer in a 1 to 200 ratio at 37 °C for 1 h. Two aneuploid blastocysts were incubated with blocking buffer without the anti-hTERT antibody to serve as a negative control. .. All blastocysts, including the negative control blastocysts, were washed 3 times in blocking buffer for a total of 30 min, and then incubated with Goat anti-Rabbit IgG 2 nd antibody (ThermoFisher Scientific, Rockford, IL, USA, Cat#35552) diluted 1:400 in blocking buffer at 37 °C for 30 min. After the second antibody incubation, blastocysts were washed three times in blocking buffer for 15 min. Immunostained blastocysts were loaded on a pre-cleaned glass slide with 15 μL of VectorShield with DAPI (Vector Laboratories, Newark, CA, USA, Cat# H-1200-10) and sealed with a coverslip and clear nail polish. .. Images were taken with a Zeiss microscope equipped with epifluorescence optics.

    Article Title: The Emerging Role of the LIV-1 Subfamily of Zinc Transporters in Breast Cancer
    Article Snippet: Fluorescent microscopy Recombinant proteins for LIV-1 ( 15 ), HKE4 ( 9 ), and ZIP14 ( 17 ) were engineered in vector pcDNA3.1/V5-His-TOPO to provide a C-terminal V5 tag as previously described. .. MCF-7 cells were seeded on coverslips for 24 h before transfection with lipofectamine 2000 as described previously ( 15 ), and after 16–24 h were prepared for fluorescence microscopy by fixing with 4% formaldehyde, permeabilizing with 0.4% saponin if required, and blocking with 10% normal goat serum before incubating with a mouse anti-V5 antibody (1/2000, Invitrogen) conjugated to Alexa Fluor (1/2000) 594 (red) or 488 (green), and then assembled onto slides using Vectorshield with DAPI (Vector Laboratories). .. All coverslips were viewed on a Leica RPE automatic microscope using a 63x oil immersion lens.

    Article Title: The Landscape of Telomere Length and Telomerase in Human Embryos at Blastocyst Stage.
    Article Snippet: Then, blastocysts were incubated in an anti-hTERT antibody (ThermoFisher Scientific, Waltham, MA, USA, Cat# PA5116024) solution diluted with blocking buffer in a 1 to 200 ratio at Genes 2023, 14, 1200 4 of 12 37 ◦C for 1 h. Two aneuploid blastocysts were incubated with blocking buffer without the anti-hTERT antibody to serve as a negative control. .. All blastocysts, including the negative control blastocysts, were washed 3 times in blocking buffer for a total of 30 min, and then incubated with Goat anti-Rabbit IgG 2nd antibody (ThermoFisher Scientific, Rockford, IL, USA, Cat#35552) diluted 1:400 in blocking buffer at 37 ◦C for 30 min. After the second antibody incubation, blastocysts were washed three times in blocking buffer for 15 min. Immunostained blastocysts were loaded on a pre-cleaned glass slide with 15 μL of VectorShield with DAPI (Vector Laboratories, Newark, CA, USA, Cat# H-1200-10) and sealed with a coverslip and clear nail polish. .. Images were taken with a Zeiss microscope equipped with epifluorescence optics.

    Incubation:

    Article Title: The Landscape of Telomere Length and Telomerase in Human Embryos at Blastocyst Stage
    Article Snippet: Then, blastocysts were incubated in an anti-hTERT antibody (ThermoFisher Scientific, Waltham, MA, USA, Cat# PA5116024) solution diluted with blocking buffer in a 1 to 200 ratio at 37 °C for 1 h. Two aneuploid blastocysts were incubated with blocking buffer without the anti-hTERT antibody to serve as a negative control. .. All blastocysts, including the negative control blastocysts, were washed 3 times in blocking buffer for a total of 30 min, and then incubated with Goat anti-Rabbit IgG 2 nd antibody (ThermoFisher Scientific, Rockford, IL, USA, Cat#35552) diluted 1:400 in blocking buffer at 37 °C for 30 min. After the second antibody incubation, blastocysts were washed three times in blocking buffer for 15 min. Immunostained blastocysts were loaded on a pre-cleaned glass slide with 15 μL of VectorShield with DAPI (Vector Laboratories, Newark, CA, USA, Cat# H-1200-10) and sealed with a coverslip and clear nail polish. .. Images were taken with a Zeiss microscope equipped with epifluorescence optics.

    Article Title: The Landscape of Telomere Length and Telomerase in Human Embryos at Blastocyst Stage.
    Article Snippet: Then, blastocysts were incubated in an anti-hTERT antibody (ThermoFisher Scientific, Waltham, MA, USA, Cat# PA5116024) solution diluted with blocking buffer in a 1 to 200 ratio at Genes 2023, 14, 1200 4 of 12 37 ◦C for 1 h. Two aneuploid blastocysts were incubated with blocking buffer without the anti-hTERT antibody to serve as a negative control. .. All blastocysts, including the negative control blastocysts, were washed 3 times in blocking buffer for a total of 30 min, and then incubated with Goat anti-Rabbit IgG 2nd antibody (ThermoFisher Scientific, Rockford, IL, USA, Cat#35552) diluted 1:400 in blocking buffer at 37 ◦C for 30 min. After the second antibody incubation, blastocysts were washed three times in blocking buffer for 15 min. Immunostained blastocysts were loaded on a pre-cleaned glass slide with 15 μL of VectorShield with DAPI (Vector Laboratories, Newark, CA, USA, Cat# H-1200-10) and sealed with a coverslip and clear nail polish. .. Images were taken with a Zeiss microscope equipped with epifluorescence optics.

    Transfection:

    Article Title: The Emerging Role of the LIV-1 Subfamily of Zinc Transporters in Breast Cancer
    Article Snippet: Fluorescent microscopy Recombinant proteins for LIV-1 ( 15 ), HKE4 ( 9 ), and ZIP14 ( 17 ) were engineered in vector pcDNA3.1/V5-His-TOPO to provide a C-terminal V5 tag as previously described. .. MCF-7 cells were seeded on coverslips for 24 h before transfection with lipofectamine 2000 as described previously ( 15 ), and after 16–24 h were prepared for fluorescence microscopy by fixing with 4% formaldehyde, permeabilizing with 0.4% saponin if required, and blocking with 10% normal goat serum before incubating with a mouse anti-V5 antibody (1/2000, Invitrogen) conjugated to Alexa Fluor (1/2000) 594 (red) or 488 (green), and then assembled onto slides using Vectorshield with DAPI (Vector Laboratories). .. All coverslips were viewed on a Leica RPE automatic microscope using a 63x oil immersion lens.

    Fluorescence:

    Article Title: The Emerging Role of the LIV-1 Subfamily of Zinc Transporters in Breast Cancer
    Article Snippet: Fluorescent microscopy Recombinant proteins for LIV-1 ( 15 ), HKE4 ( 9 ), and ZIP14 ( 17 ) were engineered in vector pcDNA3.1/V5-His-TOPO to provide a C-terminal V5 tag as previously described. .. MCF-7 cells were seeded on coverslips for 24 h before transfection with lipofectamine 2000 as described previously ( 15 ), and after 16–24 h were prepared for fluorescence microscopy by fixing with 4% formaldehyde, permeabilizing with 0.4% saponin if required, and blocking with 10% normal goat serum before incubating with a mouse anti-V5 antibody (1/2000, Invitrogen) conjugated to Alexa Fluor (1/2000) 594 (red) or 488 (green), and then assembled onto slides using Vectorshield with DAPI (Vector Laboratories). .. All coverslips were viewed on a Leica RPE automatic microscope using a 63x oil immersion lens.

    Microscopy:

    Article Title: The Emerging Role of the LIV-1 Subfamily of Zinc Transporters in Breast Cancer
    Article Snippet: Fluorescent microscopy Recombinant proteins for LIV-1 ( 15 ), HKE4 ( 9 ), and ZIP14 ( 17 ) were engineered in vector pcDNA3.1/V5-His-TOPO to provide a C-terminal V5 tag as previously described. .. MCF-7 cells were seeded on coverslips for 24 h before transfection with lipofectamine 2000 as described previously ( 15 ), and after 16–24 h were prepared for fluorescence microscopy by fixing with 4% formaldehyde, permeabilizing with 0.4% saponin if required, and blocking with 10% normal goat serum before incubating with a mouse anti-V5 antibody (1/2000, Invitrogen) conjugated to Alexa Fluor (1/2000) 594 (red) or 488 (green), and then assembled onto slides using Vectorshield with DAPI (Vector Laboratories). .. All coverslips were viewed on a Leica RPE automatic microscope using a 63x oil immersion lens.



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